Home
Introduction
Diamond Knives
ultra
cryo
cryo 25
cryo immuno
cryo AFM
cryo 35 (dry/wet)
cryo 45 (dry/wet)
trim 45 / trim 20
histo
Characteristics
Resharpening and Exchange
Quality Guarantee
Customer Service
Accessories
Distributors
News
Contact


Download the
Diatome brochure:
 ENG
 DEU
 FRA







cryo 25

The cryo 25 knife is designed for sectioning frozen hydrated specimens. The 25 angle results in the least possible compression and the best structure preservation (H.M. Han et al., Journal of Microscopy, Vol. 230, Pt. 2, pp. 167 171, 2007).

Please note: best results are achieved at low humidiy, when the cryo-ultramicrotome is placed in a glovebox and the sections attached by electrostatic force (J. Pierson et al., Journal of Structural Biology 169, pp. 219 225, 2010).
 

 
High resolution electron micrograph of vitreous section of keratin intermediate filaments in the midportion of stratum corneum of human epidermis. The fine structure of the keratin filaments is well resolved and their molecular organisation is seen in favourable cases (inset). Ashraf Al-Amoudi, Laboratoire d'Analyse Ultrastructurale, Lausanne.